SARS-CoV-2 Spike RBD Antibody [2196]

• Catalog Number: orb1274617
• Category: Antibodies
• Description: SARS-CoV-2 Spike RBD Antibody [2196]
• Clonality: Recombinant
• Clone Number: 2196
• Tested applications: ELISA
• Reactivity: Virus
• Isotype: Human IgG1
• Immunogen: Sequenced from human survivors of COVID-19 (SARS-CoV-2). The spike RBD is expressed on the surface of SARS-CoV-2.
• Concentration: > 1.0 mg/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8˚ C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80˚ C. Avoid Repeated Freeze Thaw Cycles.
• Buffer/Preservatives: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
• Alternative names: SARS-CoV-2 Spike RBD Antibody, Receptor Binding Do
• Note: For research use only
• Application notes: ELISA (Quality Tested by BIorbyt)
• Expiration Date: 12 months from date of receipt.

Coating: Purified Recombinant SARS-CoV-2 Spike NTD (Clone 2146) was coated at concentration of 1 ul/well, 100 ul/well overnight 2-8 ˚C. Sample: Purified rSARS CoV-2 Trimeric Spike Protein at 0.5 µg/mL and serially diluted (2-fold) to 0.98 ng/ml, 100ul/well for 1 hour at 37 ˚C. Detection: Anti-SARS-CoV-2 Spike RBD (Clone 2196) conjugated to HRP diluted to 0.1 ug/ml, 100 ul/well for 1 hour at 37 ˚C. Substrate: TMB, 100 ul/well for 15 min, at room temperature followed by 450 nm stop solution, 50ul/well.