SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb Antibody

• Catalog Number: orb1945971
• Category: Antibodies
• Description: SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb Antibody
• Clonality: Monoclonal
• Clone Number: CoV2-CA11
• Tested applications: FA
• Reactivity: Other
• Isotype: Rabbit IgGk
• Immunogen: recombinant protein (RBD)
• Concentration: 0.5 mg/ml
• Dilution range: This antibody recognizes the SARS-CoV-2 Spike Protein RBD domain and inhibits the interaction between SARS-CoV-2 RBD and ACE2. Can be paired with other antibody for detection in sandwich ELISA format.
• Conjugation: Unconjugated
• UniProt ID: P0DTC2
• Storage: -20°C
• Buffer/Preservatives: 1X PBS
• Alternative names: Spike protein S1, Spike protein S2, RBD domain, Bi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry neutralizing assay: C-A11 displayed dose dependent blocking of spike proteins (wild type and UK variant) to ACE2 expressing cells.

Affinity assessment of SARS-CoV-2 C-A11 IgG to wild type and B.1.1.7 (UK) S1 + S2 trimers. C-A11 possesses very low (pM) KD affinity to both variants.

Flow cytomery neutralizing assay of C-A11 (plotted graphs): ACE2 transfected cells were incubated with biotinylated spike protein (0.5 ug/mL) in the presence or absence of antibody. Binding of spike protein was detected with DyLight488 conjugated StreptAv

Binding of C-A11 to SARS-CoV-2 variants (UK and South Africa): C-A11 shows good cross reactivity to both variants B.1.1.7 (UK) and B.1.351 (S. Africa).