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Item 1 of 4
SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb
Catalog Number: orb1945971
| Catalog Number | orb1945971 |
|---|---|
| Category | Antibodies |
| Description | SARS-CoV-2 spike protein (RBD) neutralizing (C-A11) rabbit mAb |
| Clonality | Monoclonal |
| Clone Number | CoV2-CA11 |
| Tested applications | FA |
| Reactivity | Other |
| Isotype | Rabbit IgGk |
| Immunogen | recombinant protein (RBD) |
| Concentration | 0.5 mg/ml |
| Dilution range | This antibody recognizes the SARS-CoV-2 Spike Protein RBD domain and inhibits the interaction between SARS-CoV-2 RBD and ACE2. Can be paired with other antibody for detection in sandwich ELISA format. |
| Conjugation | Unconjugated |
| UniProt ID | P0DTC2 |
| Storage | -20°C |
| Buffer/Preservatives | 1X PBS |
| Alternative names | Spike protein S1, Spike protein S2, RBD domain, Bi Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Flow cytometry neutralizing assay: C-A11 displayed dose dependent blocking of spike proteins (wild type and UK variant) to ACE2 expressing cells.
Affinity assessment of SARS-CoV-2 C-A11 IgG to wild type and B.1.1.7 (UK) S1 + S2 trimers. C-A11 possesses very low (pM) KD affinity to both variants.
Flow cytomery neutralizing assay of C-A11 (plotted graphs): ACE2 transfected cells were incubated with biotinylated spike protein (0.5 ug/mL) in the presence or absence of antibody. Binding of spike protein was detected with DyLight488 conjugated StreptAv
Binding of C-A11 to SARS-CoV-2 variants (UK and South Africa): C-A11 shows good cross reactivity to both variants B.1.1.7 (UK) and B.1.351 (S. Africa).
