SARS-CoV-2 Spike NTD Antibody [2146]

• Catalog Number: orb1274626
• Category: Antibodies
• Description: SARS-CoV-2 Spike NTD Antibody [2146]
• Clonality: Recombinant
• Clone Number: 2146
• Tested applications: ELISA, IHC
• Reactivity: Virus
• Isotype: Human IgG1
• Immunogen: Sequenced from human survivors of COVID-19 (SARS-CoV-2). The spike NTD is expressed on the surface of SARS-CoV-2.
• Concentration: > 1.0 mg/ml
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Functional grade preclinical antibodies may be stored sterile as received at 2-8˚ C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80˚ C. Avoid Repeated Freeze Thaw Cycles.
• Buffer/Preservatives: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
• Alternative names: SARS-CoV2 Spike NTD, COVID-19, 2019-nCoV, Severe a
• Note: For research use only
• Application notes: ELISA (Quality Tested by BIorbyt); IHC (Other applications as reported in literature)
• Expiration Date: 12 months from date of receipt.

Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8 ˚C. Detection: Anti-SARS-CoV-2 NTD (Clone 2146) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37 ˚C. Substrate: TMB (Leinco T118), 100 ul/well for 15 min, at room temperature followed by 450 nm stop solution, 50ul/well.