SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5]

• Catalog Number: orb1274188
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike S2 Single Domain Antibody [P1G5]
• Species/Host: Camelus
• Clonality: Recombinant
• Clone Number: P1G5
• Tested applications: ELISA
• Reactivity: Virus
• Isotype: sdAb
• Immunogen: SARS-CoV-2 Spike S2 protein containing C-terminal His Tag. The protein was expressed in human 293 cells (HEK293). It contains amino acids Ser 686 - Pro 1213.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• NCBI: QHD43416
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Spike S2 Antibody is supplied in PBS.
• Alternative names: SARS-CoV-2 (COVID-19) Spike S2 Antibody: Severe ac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Validation with Spike Trimer Proteins of SARS-CoV-2 Variants and SARS-CoV-1 Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1274188. A direct ELISA was performed using Spike trimer protein of SARS-CoV-2 Variants (Delta and Omicron) and SARS-CoV-1 as coating antigens at 1 μg/mL and the anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (orb1274188) as the capture antibody, following by anti-cMyc-tag antibody (orb1239964) at 1 μg/mL. Secondary: Goat anti-mouse IgG HRP conjugate at 1:5000 dilution. Detection range is from 16 ng/mL to 2000 ng/mL.

Epitope Mapping of four Spike S2 Antibodies by Direct Competitive ELISA A direct competitive ELISA was developed for the epitope mapping using Spike trimer proteins of SARS-CoV-2 Delta variant at 1 μg/ml as the coating antigen and biotinylated Spike S2 single domain antibodies (sdAbs) as the competitive antibodies. The sdAb mixtures (biotinylated and unconjugated sdAbs) were added to ELISA plate and streptavidin-HRP was used to detect the captured biotinylated sdAbs. The assay suggests the antibodies bind to different epitopes of the Spike trimer.