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Catalog Number | orb1274035 |
---|---|
Category | Antibodies |
Description | SARS-CoV-2 (COVID-19) Spike S2 Antibody (CT) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Virus |
Isotype | IgG |
Immunogen | Synthetic peptide located within the C terminus of SARS-CoV-2 (COVID-19) Spike S1. |
Antibody Type | Primary Antibody |
Concentration | batch dependent |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | S |
NCBI | QHD43416.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 10% glycerol, pH 7.2 |
Alternative names | SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Antibody: S Read more... |
Note | For research use only |
Application notes | WB: 1:500-1000 |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation with Recombinant Protein. Loading: 1 ug of recombinant protein per lane. Antibodies: SARS-CoV-2 (COVID-19) Spike S2, orb1274035, 1:500. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution.
ELISA Test. Coating original concentration: 2 ug/mL, 100 uL/well samples are column 1: SARS-CoV-2 (COVID-19) Spike-ECD Recombinant Protein, and column 2: SARS-CoV-2 (COVID-19) Spike-RBD Recombinant Protein, orb1231624. Antibodies: SARS-CoV-2 (COVID-19) Spike S2 Antibody, orb1274035. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Develop: 15 min, 100 uL/well. Stop: Stop buffer 50 uL/well.