SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant)

• Catalog Number: orb1238696
• Category: Tools
• Description: SARS-CoV-2 (COVID-19) Spike P681R Antibody [7E3C5] (Delta Variant)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, IHC-P
• Isotype: IgG2
• Immunogen: Anti-SARS-CoV-2 Spike 681R (Delta Variant) antibody (orb1238696) was raised against a peptide corresponding to 12 amino acids near the cleavage site of SARS-CoV-2 Delta variant Spike protein. The immunogen is located within 650-700 amino acids of SARS-CoV-2 Delta variant Spike protein.
• Concentration: 1 mg/mL
• Dilution range: IHC: 20 μg/mL. Antibody validated: IHC in human samples. Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects SARS-CoV-2 Delta Variant Spike S1 protein, but not SARS-CoV-2 WT and other variant Spike S1 protein by ELISA. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike P681R (Delta Variant) antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 Spike P681R (Delta Variant) antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Anti-SARS-CoV-2 Spike P681R (Delta Variant) antibo
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of Spike in Delta Variant COVID-19 Patient Lung Tissue.Immunohistochemical analysis of paraffin-embedded patient lung tissue using anti-Spike P681R antibody (orb1238696) at 20 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in the Delta variant COVID-19 patient lung, but not in WT COVID-19 patient lung.

SARS-Cov-2 Spike P681R (Delta Variant) Antibodies Specifically Detect Delta Variant Spike S1 Protein in an ELISA.Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, orb1238696, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr.

ELISA Validation of Delta Variant Spike S1 Antibodies with SARS-CoV-2 Delta Variant Spike S1 Protein.Coating Antigen: SARS-CoV-2 spike S1 proteins, WT, Alpha and Delta variants, 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike P681R (Delta Variant) antibody, orb1238696, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody specifically detects spike S1 protein of Delta variant, but not WT and Alpha variant.

ELISA Validation of Delta Variant Spike S1 Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231701), Alpha variant (orb1231664), and Delta variant (orb1231658), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 delta spike P681R antibody, orb1238696, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike P681R (Delta Variant) antibody detects Delta variant spike S1 peptide (681R, orb1231658), but not WT peptide (681P, orb1231701) and Alpha variant spike S1 peptide (681H, orb1231664).