SARS-CoV-2 (COVID-19) Spike G142D Δ143-145VYY Antibody (Omicron)

• Catalog Number: orb1239983
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike G142D Δ143-145VYY Antibody (Omicron)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 Omicron variant specific Spike (G142D Δ143-145VYY) antibody(orb1239983) was raised against a peptide corresponding to 12 amino acids near the amino terminal domain of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein. The immunogen is located within 130-180 amino acids of SARS-CoV-2 Omicron variant (B.1.1.529) Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 2 μg/mL; ICC: 1 μg/mL; IF: 10 μg/mL. Antibody validated: Western Blot, Immunocytochemistry and Immunofluorescence staining in human samples. Anti-SARS-CoV-2 Omicron variant Spike antibody specifically detects Spike S1 protein of Omicron Variant (B.1.1.529), but not WT and other variant by ELISA and WB. Other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Omicron Variant Spike antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Anti-SARS-CoV-2 Omicron Variant Spike antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Omicron Variant Spike antibody: Severe
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Omicron COVID-19 Patient. Immunohistochemical analysis of 4% paraformaldehyde-fixed cells from a nasal swab from a patient infected with the Omicron variant stained with Omicron specific antibody (orb1239983) at 1 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of Omicron Variant Spike Specific Antibody on a Nasal Swab Sample from a Patient Infected with the Omicron Variant. Immunofluorescent analysis of 4% paraformaldehyde-fixed patient nasal swab sample labeling spike with orb1239983 and nucleocapsid with orb1273019 at 10 µg/mL for each, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution for spike (orb1239983