SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1]

• Catalog Number: orb1239047
• Category: Tools
• Description: SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10B1]
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, WB
• Isotype: IgG3
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike 681P antibody (orb1239047) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Spike protein. The immunogen is located within 650-700 amino acids of the SARS-CoV-2 Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL.Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 681P antibody can detect SARS-CoV-2 WT Spike S1 protein, but not SARS-CoV-2 Alpha Variant (B.1.1.7, UK) S1 protein by ELISA and WB. It can also detect WT peptide (681P), but not mutant peptide (681H). All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike 681P antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Anti-SARS-CoV-2 Spike 681P antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Spike 681P antibody: Severe acute respi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein.Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike 681P antibody, orb1239047, dilution: 1-3000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239047) detected WT spike S1 protein (orb1231589), but not alpha variant spike S1 protein by ELISA.

WB Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein.Loading: 30 ng of SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike 681P antibody, orb1239047, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239047) detected WT spike S1 protein (orb1231589), but not alpha variant spike S1 protein by WB.

ELISA Validation of Spike 681P Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231701) and alpha variant (B.1.1.7, UK) (orb1231664), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 681P antibody, orb1239047, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239047) detected WT peptide (681P, orb1231701), but not alpha variant spike S1 peptide (681H, orb1231664).