SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9]

• Catalog Number: orb1238713
• Category: Tools
• Description: SARS-CoV-2 (COVID-19) Spike 26P Antibody [1C3H9]
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, WB
• Isotype: IgG2b
• Immunogen: Anti-SARS-CoV-2 Spike 26P antibody (orb1238713) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Spike protein.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL. Antibody validated: Western Blot in human samples. Anti-SARS-CoV-2 Spike 26P antibody detects all other SARS-CoV-2 variant Spike S1 protein, but not SARS-CoV-2 Gamma variant Spike S1 protein by ELISA. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: SARS-CoV-2 Spike 26P antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: SARS-CoV-2 Spike 26P antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Spike 156-157EF antibody: Severe acute
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

SARS-Cov-2 Spike 26P Antibodies Detect Spike S1 Proteins of all Variants except Gamma Variant in an ELISA.Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, orb1238713, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubate at RT for 1 hr.

ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein.Coating Antigen: SARS-CoV-2 spike S1 proteins of WT and Gamma variant (P.1), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike 26P antibody, orb1238713, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (orb1231589), but not Gamma variant spike S1 protein.

ELISA Validation of Spike 26P Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein.Loading: 50 ng of SARS-CoV-2 spike S1 proteins, WT and Gamma variant (P.1). Detection Antibodies: Spike 26P antibody, orb1238713, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 Spike 26P antibody detects WT spike S1 protein (orb1231589), but not Gamma variant spike S1 protein.

ELISA Validation of Spike 26P Antibodies with Mutant and WT Peptide. Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231703) and Gamma variant (P.1) (orb1231659), 1 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 26P antibody, orb1238713, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 26P antibody detects WT peptide (26P, orb1231703), but not Gamma variant spike S1 peptide (26S, orb1231659).