SARS-CoV-2 (COVID-19) nucleocapsid

• Catalog Number: orb1294283
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SARS-CoV 2 nucleocapsid protein
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, NeA, WB
• Reactivity: Virus
• Isotype: Rabbit-IgG
• Immunogen: Recombinant fragment corresponding to SARS-CoV 2 nucleocapsid protein
• Dilution range: Western Blot 1:3000-1:5000 Immunofluorescence 1:500-1:1000
• Conjugation: Unconjugated
• Target: SARS-CoV-2 (COVID-19) nucleocapsid
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-SARS-CoV-2 nucleocapsid protein antibody at 1/3000 dilution, Lysates/proteins at 30 μg per lane. This blot was produced using a 12% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294283 overnight at 4°C.

Immunofluorescence: Cells were stained with orb1294283 anti-SARS-CoV-2 nucleocapsid protein antibody (green) at 1:500 and 4°C. DAPI (blue) was used as the nuclear counter stain.