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SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (HRP)
Catalog Number: orb1239066
| Catalog Number | orb1239066 |
|---|---|
| Category | Antibodies |
| Description | SARS-CoV-2 (COVID-19) Nucleocapsid Antibody (HRP) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Virus |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (HRP) (9099) was raised against a peptide corresponding to 17 amino acids near the center of SARS-CoV-2 (COVID-19) Nucleocapsid protein. The immunogen is located within 230-280 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | HRP |
| Target | N |
| NCBI | QHD43423 |
| Storage | SARS-CoV-2°COVID-19) Nucleocapsid antibody can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | SARS-CoV-2 (COVID-19) Nucleocapsid antibody: Sever Read more... |
| Note | For research use only |
| Application notes | IHC: 1 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid Protein in COVID-19 Patient Lung.Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) nucleocapsid antibody (orb1239066, 2 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 nucleocapsid protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation.Coating Antigen: immunogen peptide, orb1231699, 10 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239066 or orb1239972, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239972 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10, 000, incubate at RT for 1 hr.
