SARS-CoV-2 (COVID-19) NSP5 Recombinant Protein

• Catalog Number: orb1231120
• Category: Proteins
• Description: SARS-CoV-2 (COVID-19) NSP5 Recombinant Protein
• Tag: N-Term GST Cleaved, C-Term 6His Cleaved
• Concentration: batch dependent
• Form/Appearance: Liquid
• Purity: Cobalt Agarose (0.9)
• MW: Mono-Isotopic Mass: 33, 796.64 daltons after tag cleavageAverage Mass: 33, 774.50 daltons after tag cleavage
• Target: ORF1ab
• UniProt ID: P0DTC1
• Protein Sequence: Native Sequence:MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQAmino acids S1 – Q306 (end).Residue S237 of the fusion protein is equivalent to S1 of the native enzyme.The GST tag is located at residues 1 – 220 and the His6 tag is located at residues 545– 550.Has C-terminal 5’ residues of NSP4 (residues 232 – 236) between PreScission site and N-terminus of NSP5 – corresponding to the cleavage site between NSP4 and NSP5 in the polyprotein of the SARS CoV virus to generate an authentic N-terminus during gene expression. The C-terminus encodes for a modified PreScission cleavage site before the His6 tag to generate an authentic C-terminus when cleaved, SGVTFQGP, residues 537 - 544.Protease Cleavage:Modified PreScission - SGVTFQGP, residues 537 - 544
• Source: bacteria
• NCBI: QHD43415.1
• Storage: Store at -70 °C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35
• Alternative names: SARS-CoV2-NSP5, SARS-CoV-2 NSP5, NSP5 SARS-CoV-2,
• Note: For research use only
• Expiration Date: 6 months from date of receipt.