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SARS-CoV-2 (COVID-19) NSP2 Antibody
Catalog Number: orb1239074
| Catalog Number | orb1239074 |
|---|---|
| Category | Antibodies |
| Description | SARS-CoV-2 (COVID-19) NSP2 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Virus |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) NSP2 antibody (orb1239074) was raised against a peptide corresponding to 15 amino acids near the center of SARS-CoV-2 (COVID-19) NSP2 protein. The immunogen is located within 50-100 amino acids of the SARS-CoV-2 (COVID-19) NSP2 protein. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| Target | Non-structural protein 2 |
| UniProt ID | P0DTC1 |
| NCBI | YP_009742609.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) NSP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | NSP2, p65 homolog Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP2 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP2 antibody (orb1239074, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP2 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation with SARS-CoV-2 (COVID-19) NSP2 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP2 Antibody, orb1239074 A direct ELISA was performed using SARS-CoV-2 NSP2 recombinant protein (orb1231565) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP2 antibody as the capture antibody Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
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