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Catalog Number | orb1239076 |
---|---|
Category | Antibodies |
Description | SARS-CoV-2 (COVID-19) NSP15 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Virus |
Isotype | IgG |
Immunogen | Anti-SARS-CoV-2 (COVID-19) NSP15 antibody (orb1239076) was raised against a peptide corresponding to 15 amino acids near the carboxyl terminus of SARS-CoV-2 (COVID-19) NSP15 protein. The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) NSP15 protein. |
Antibody Type | Primary Antibody |
Concentration | 1 mg/mL |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | NSP15 |
UniProt ID | P0DTC1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | SARS-CoV-2 (COVID-19) NSP15 antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | Uridylate-specific endoribonuclease, NendoU, NSP15 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
ELISA Validation with SARS-CoV-2 (COVID-19) NSP15 Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP15 Antibody, orb1239076. A direct ELISA was performed using SARS-CoV-2 NSP15 recombinant protein (orb1231570) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP15 antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.
Western Blot Validation with SARS-CoV-2 (COVID-19) NSP15 Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) NSP15 recombinant protein (orb1231570). Antibodies: SARS-CoV-2 (COVID-19) NSP15, orb1239076, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL and. Lane 2: 1 µg/mL.