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Catalog Number | orb1231059 |
---|---|
Category | Proteins |
Description | SARS-CoV-2 (COVID-19) NSP10 Recombinant Protein |
Tag | N-Term GST Uncleaved |
Concentration | batch dependent |
Form/Appearance | Liquid |
Purity | GSH-Agarose (0.6) |
MW | Mono-Isotopic Mass: 41, 586.44 daltons Average Mass: 41, 614.09 daltons |
Target | ORF1ab |
UniProt ID | P0DTC1 |
Protein Sequence | Native Sequence:MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQAmino acids A1 – Q139 (end).Residue A232 of the fusion protein is equivalent to A1 of the native enzyme.The GST tag is located at residues 1 – 220.Protease Cleavage:PreScission (LEVLFQGP) residues 221 - 228 |
Source | bacteria |
NCBI | QHD43415.1 |
Storage | Store at -70 °C. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | 50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35 |
Alternative names | SARS-CoV2-NSP10, SARS-CoV-2 NSP10, NSP10 SARS-CoV- Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 90% as determined by SDS-PAGE quantitative densitometry by Coomassie Blue Staining. | |
17.99kDa | |
E. coli |
Amylose Resin (0.6) | |
Mono-Isotopic Mass: 59, 810.55 daltons Average Mass: 59, 848.71 daltons | |
bacteria |
≥ 90% as determined by SDS-PAGE | |
Recombinant SARS-CoV-2 NSP10 was expressed from E.coli expression system and the target gene encoding Ala1-Gln139 is expressed with N-His Tag |