SARS-CoV-2 (COVID-19) Membrane Antibody (biotin)

SKU: orb1239081

Description

SARS-CoV-2 (COVID-19) Membrane Antibody (biotin)

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Virus
• Application Notes:
  IHC: 2 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Membrane antibody (biotin) was raised against a peptide corresponding to 13 amino acids near the center of SARS-CoV-2 (COVID-19) Membrane protein. The immunogen is located between 130-180 amino acids of the SARS-CoV-2 (COVID-19) Membrane protein.
• Target: M
• Purification: SARS-CoV-2 (COVID-19) Membrane Antibody is affinity chromatography

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Membrane Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

SARS-CoV-2/SARS-CoV Matrix Antibody: SARS-CoV-2 Matrix protein, SARS Matrix protein, Membrane protein, E1 glycoprotein, M protein

ELISA Validation.Coating Antigen: immunogen peptide, orb1231692, 10 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239081 or orb1239084, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239081 was detected by HRP-conjugated streptavidin at 1:5000 and orb1239084 was detected by anti-rabbit HRP conjugated secondary antibodies at 1:10, 000, incubate at RT for 1 hr.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Membrane Protein in COVID-19 Patient Lung.Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) membrane antibody (orb1239083, 2 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C, following by streptavidin-HRP conjugate at 20 μg/mL. Counter stained with Hematoxylin. SARS-COV-2 membrane protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Quick Database Links

Gene Symbol

M

UniProt

P0DTC5

RefSeq:QJW69073