SARS-CoV-2 (COVID-19) Envelope Antibody (HRP)

SKU: orb1239043

Description

SARS-CoV-2 (COVID-19) Envelope Antibody (HRP)

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Virus
• Application Notes:
  IHC: 2 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody (HRP) was raised against a peptide corresponding to 10 amino acids near the amino terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope.
• Target: E
• Purification: SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody is affinity chromatography purified via peptide column.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Envelope Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Envelope protein, E protein

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Envelope Protein in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) envelope antibody (orb1239043, 2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation. Coating Antigen: immunogen peptide, orb1236593, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239043 or orb1239971, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239971 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.

Quick Database Links

Gene Symbol

E

UniProt

QHD43418

RefSeq:QHD43418