SARS-CoV-2 Human Neutralizing mAb Antibody

SKU: orb1945952

Description

SARS-CoV-2 6R8A-BG33 Human Neutralizing mAb

Research Area

Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Dilution Range: Virus neutralization
• Reactivity: Other

Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG1k
• Clone No.: BG33
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 1X PBS
• Concentration: 0.5 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

The microtiter wells were coated with recombinant ACE2-Fc protein at 2 μg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxidase conjugated rabbit anti-His tag antibody and the inhibition of binding (IC50 ng/mL) was calculated using Prism 9 software. The experiments were performed in triplicates.

The microtiter wells were coated with recombinant ACE2-Fc protein at 2 μg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxidase conjugated rabbit anti-His tag antibody and the inhibition of binding (IC50 ng/mL) was calculated using Prism 9 software. The experiments were performed in triplicates.