SARS-CoV-2 6R8A-E11 human neutralizing mAb Antibody

• Catalog Number: orb1945952
• Category: Antibodies
• Description: SARS-CoV-2 6R8A-E11 human neutralizing mAb Antibody
• Clonality: Monoclonal
• Clone Number: 6R8A-E11
• Tested applications: ELISA
• Reactivity: Other
• Isotype: Human IgG1k
• Concentration: 0.5 mg/ml
• Dilution range: Virus neutralization
• Conjugation: Unconjugated
• Storage: -20°C
• Buffer/Preservatives: 1X PBS
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The microtiter wells were coated with recombinant ACE2-Fc protein at 2 μg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxidase conjugated rabbit anti-His tag antibody and the inhibition of binding (IC50 ng/mL) was calculated using Prism 9 software. The experiments were performed in triplicates.

The microtiter wells were coated with recombinant ACE2-Fc protein at 2 μg/mL. The spike trimer protein (96 ng/mL) was mixed with serially diluted antibody 1:1 at RT for 1 hour and added to the wells. Bound spike trimer was detected with horseradish peroxidase conjugated rabbit anti-His tag antibody and the inhibition of binding (IC50 ng/mL) was calculated using Prism 9 software. The experiments were performed in triplicates.