soxA Antibody (Biotin)

SKU: orb344281

Description

Sarcosine Oxidase antibody (Biotin)

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:1,000 - 1:2,500, IP: 1:100, WB: 1:500 - 1:1,000
• Application Notes:
  Anti-Sarcosine Oxidase Biotin Conjugated Antibody has been tested by ELISA and western blot. This product is suitable to be assayed against 1.0 ug of Sarcosine Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Reconstitution Buffer: Restore with deionized water (or equivalent). Reconstitution Volume: 100 µL

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Sarcosine Oxidase [Microbial]
• Target: soxA
• Purity: Sarcosine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Sarcosine Oxidase [Microbial]. Cross reactivity against Sarcosine Oxidase from other sources is unknown.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

goat anti-Sarcosine Oxidase Antibody, biotin Conjugated goat anti-Sarcosine Oxidase Antibody, Sarcosine oxidase subunit alpha, Sarcosine oxidase subunit

Biorbyt Goat anti Sarcosine Oxidase antibody was used to detect Sarcosine Oxidase. Samples of ~1 and 0.25 ug of purified Sarcosine Oxidase per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Biorbyt Goat anti Sarcosine Oxidase. Primary antibody was detected with Biorbyt Dylight 649 conjugated Donkey anti Goat (1:10K 1.5 hr RT in orb348637).

Western Blot of Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated. Lane 1: Sarcosine Oxidase (Microbial). Load: 50 ng per lane. Primary antibody: Goat anti-Sarcosine Oxidase (Microbial) Antibody Biotin Conjugated at 1:1000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 45 kDa, 45 kDa for Sarcosine Oxidase monomeric alpha subunit.

Quick Database Links

Gene Symbol

soxA

UniProt

Q46337

RefSeq:YP_004446819.1