Sal-like 4 Antibody / SALL4

SKU: orb1824101

Description

Sall3 (SALL3, sal-like 3) and Sall4 (SALL4, sal-like 4) are mammalian homologs of the Drosophila region-specific homeotic gene spalt, which encodes a zinc finger-containing transcription regulator. Drosophila spalt is an essential genetic component required for the specification of posterior head and anterior tail as opposed to trunk. Sall3 is expressed at 24 weeks of gestation in several regions of the human fetal brain including neurons of the hippocampus formation and of mediodorsal and ventrolateral thalamic nuclei, Purkinje cells of the cerebellum, and a subset of neurons in the brainstem. Sall4 expression in early mouse embryos is gradually confined to the head region and the primitive streak, followed by prominent expression in the developing midbrain, branchial arches, limbs and genital papilla.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Sal-like 4 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: SALL4/7802R
• Immunogen: A recombinant partial protein sequence (within amino acids 100-300) from the human protein was used as the immunogen for the Sal-like 4 antibody.
• Purification: Protein A affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human kidney cancer with Sal-like 4 antibody (clone SALL4/7802). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human seminoma tissue with Sal-like 4 antibody (clone SALL4/7802). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q9UJQ4