Sal003

SKU: orb1223414

Description

SAL-003 is a cell-permeable inhibitor of eIF2α dephosphorylation. SAL003 is an Analog of salubrinal (Cat. No. 2347) with improved aqueous solubility. Sal003 mediated upregulation of eIF2α phosphorylation suppressed cisplatin induced p53 activation. Furthermore, reduction of eIF2α phosphorylation by PERK knockdown enhanced cisplatin-induced p53 activation and apoptosis.

Key Properties

• CAS Number: 1164470-53-4
• MW: 463.21
• Purity: >98% (HPLC)
• Formula: C₁₈H₁₅Cl₄N₃OS
• SMILES: c1ccc(cc1)/C=C/C(=O)NC(C(Cl)(Cl)Cl)NC(=S)Nc1ccc(cc1)Cl
• Target: Calcium Channel
• Solubility: DMSO : ≥ 100 mg/mL 215.88 mM

Bioactivity

• In Vivo:
  Animal model: Rats (300-325g). Dosage: 20 μM. Administration: Intrahippocampal injection; 8 minutes. Result: Impaired contextual memory.
• In Vitro:
  Apoptosis Analysis Cell line: HeLa cells. Concentration: 10 μM. Incubation time: 1 hour. Result: Phosphorylated eIF2α and thus enhanced SubAB-induced apoptotic signaling. Western blot analysis. Cell line: Mouse embryonic fibroblasts (MEFs). Concentration: 20 μM. Incubation time: 1 hour, 3 hours, 6 hours, 12 hours. Result: Sharply increased eIF2α phosphorylation in mouse MEFs.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SAL-003 | SAL 003 | SAL003

Quick Database Links

Gene Symbol

Calcium Channel