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Catalog Number | orb1257718 |
---|---|
Category | Antibodies |
Description | SAFB2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-255 of human SAFB2 (NP_055464.1). |
Concentration | batch dependent |
Dilution range | WB: 1:1000 - 1:2000IHC: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 160kDa |
Target | SAFB2 |
UniProt ID | Q14151 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | KIAA0138, scaffold attachment factor B2, SAFB2, KI Read more... |
Note | For research use only |
Application notes | WB: 1:1000 - 1:2000IHC: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SAFB2 antibody (orb1257718) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human prostate using SAFB2 antibody (orb1257718) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using SAFB2 antibody (orb1257718) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SAFB2 antibody (orb1257718) at dilution of 1:100 (40x lens).
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