Recombinant S100P Antibody / MIG9

SKU: orb2636081

Description

S100P is a 95-amino-acid protein and a member of the S100 family. S100P has been shown to mediate tumor growth, metastasis and invasion through the binding of Ca2+ ions, receptor for advanced glycation end products, cytoskeletal protein ezrin, calcyclin-binding protein/Siah-1-interacting protein and cathepsin D. S100P highly expressed in human placenta, gastrointestinal tract, and esophageal mucosa, but always negative in pancreas and liver. Overexpression of S100P has been detected in several cancers such as breast, colon, prostate, pancreatic and lung carcinomas, and the protein has been functionally implicated in carcinogenic processes. S100P could potentially serve as diagnostic marker, prognostic/predictive indicator and therapy target for different carcinomas.

Images & Validation

• Tested Applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the recombinant S100P antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG2b, kappa
• Clone No.: rS100P/9254
• Immunogen: A recombinant fragment corresponding to the C-terminus of human S100P/MIG9 protein was used as the immunogen for the recombinant S100P antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC staining of FFPE human placental tissue with recombinant S100P antibody (clone rS100P/9254). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant S100P antibody (clone rS100P/9254) as confirmation of integrity and purity.

Quick Database Links

UniProt

P25815