MRP14 Antibody / S100A9

SKU: orb2639166

Description

Recognizes a protein of14 kDa, identified as MRP-14 (also known as Calgranulin B, S100A9 and Calprotectin L1H). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This mAb reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE; not suitable for frozen tissues): 0.5-1ug/ml for 30 min at RT (1)
• Reactivity: Human
• Application Notes:
  Optimal dilution of the MRP14 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgM, kappa
• Clone No.: MRP14/840
• Immunogen: Recombinant human protein was used as the immunogen for the MRP14 antibody.
• Purification: PEG precipitation
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with MRP14 antibody (MRP14/840)

Quick Database Links

UniProt

P06702