S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

• Catalog Number: orb750899
• Category: Antibodies
• Description: Mouse monoclonal antibody against S100A4 conjugated to Biotin
• Target: S100A4
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: Biotin
• Reactivity: Human, Mouse
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Concentration: Purified Ab conjugated to Biotin
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Purification: Protein G
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• UniProt ID: P26447
• MW: 10-12kDa
• Tested applications: FC, IF, IHC, WB
• Dilution range: Flow Cytometry (5ul/million cells); Western Blot (2-4ug/ml); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Application notes: Positive Control: HeLa, A549 or A375 cells. Human placenta tissue.
• Clone Number: S100A4/1481
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-S100 calcium-binding protein A4 antibody, ant
• Research Area: Immunology & Inflammation
• Note: For research use only
• Entrez: 6275
• Expiration Date: 12 months from date of receipt.

Analysis of Protein Array containing more than 19000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (S100A4/1481). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow Cytometric Analysis of A549 cells using S100A4 Mouse Monoclonal Antibody (S100A4/1481) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of T98G cells using S100A4 Mouse Monoclonal Antibody (S100A4/1481) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (S100A4/1481). Confirmation of Integrity and Purity of Antibody.