S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

SKU: orb750899

Description

Mouse monoclonal antibody against S100A4 conjugated to Biotin

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution range: Flow Cytometry (5ul/million cells); Western Blot (2-4ug/ml); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human, Mouse
• Application Notes:
  Positive Control: HeLa, A549 or A375 cells. Human placenta tissue.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: S100A4/1481
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Target: S100A4
• Source/Expression System: Bioreactor
• Molecular Weight: 10-12kDa
• Purification: Protein G
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Disclaimer: For research use only

Alternative Names

Anti-S100 calcium-binding protein A4 antibody, anti-CAPL antibody, anti-Fibroblast specific protein 1 (FSP1) antibody, anti-Leukemia multidrug resistance associated protein antibody, anti-Malignant transformation suppression 1 (MTS1) antibody, anti-Metastasin antibody, anti-Placental calcium-binding protein antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using S100A4 Mouse Monoclonal Antibody (S100A4/1481). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow Cytometric Analysis of A549 cells using S100A4 Mouse Monoclonal Antibody (S100A4/1481) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of T98G cells using S100A4 Mouse Monoclonal Antibody (S100A4/1481) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

SDS-PAGE Analysis Purified S100A4 Mouse Monoclonal Antibody (S100A4/1481). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

S100A4

UniProt

P26447

NCBI Gene ID

6275