S100A4 Antibody (Biotin)

SKU: orb750899

Description

Mouse monoclonal antibody against S100A4 conjugated to Biotin

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Flow Cytometry (5ul/million cells); Western Blot (2-4ug/ml); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human, Mouse
• Application Notes:
  Positive Control: HeLa, A549 or A375 cells. Human placenta tissue.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: S100A4/1481
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Target: S100A4
• Source/Expression System: Bioreactor
• Molecular Weight: 10-12kDa
• Purification: Protein G
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-S100 calcium-binding protein A4 antibody, anti-CAPL antibody, anti-Fibroblast specific protein 1 (FSP1) antibody, anti-Leukemia multidrug resistance associated protein antibody, anti-Malignant transformation suppression 1 (MTS1) antibody, anti-Metastasin antibody, anti-Placental calcium-binding protein antibody

Quick Database Links

Gene Symbol

S100A4

UniProt

P26447

NCBI Gene ID

6275