S100A4 Antibody / FSP1

SKU: orb639913

Description

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/million cells in 0.1ml,Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Reactivity: Human, Mouse
• Application Notes:
  Optimal dilution of the antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG1, kappa
• Clone No.: rS100A4/1481
• Immunogen: A recombinant human partial protein (amino acids 1-200) was used as the immunogen for the recombinant FSP1 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

IHC testing of FFPE human placenta with FSP1 antibody (clone rS100A4/1481). Required HIER: steam sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling.

IF/ICC testing of fixed and permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481, green) and Reddot nuclear stain (red).

Flow cytometry testing of permeabilized human A549 cells with FSP1 antibody (clone rS100A4/1481); Red=isotype control, Blue= FSP1 antibody.

Western blot testing of human PANC-1 cell lysate with FSP1 antibody. Predicted molecular weight ~12 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant FSP1 antibody (clone rS100A4/1481) as confirmation of integrity and purity.

Quick Database Links

UniProt

P26447