S100A16 Antibody

SKU: orb1824440

Description

S-100A16 (S100 calcium binding protein A16), also known as AAG13 (aging-associated gene 13 protein), S100F or DT1P1A7, is a 103 amino acid nuclear and cytoplasmic protein that exists as a homodimer that binds one calcium ion per monomer. A member of the EF-hand superfamily, S-100A16 contains two EF-hand domains and is encoded by a gene that maps to human chromosome 1q21.3.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the S100A16 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2, kappa
• Clone No.: S100A16/7412
• Immunogen: A recombinant partial protein sequence (within amino acids 1-103) from the human protein was used as the immunogen for the S100A16 antibody.
• Purification: Protein A/G affinity
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human skin tissue with S100A16 antibody (clone S100A16/7412). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human duodenum tissue with S100A16 antibody (clone S100A16/7412). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma tissue with S100A16 antibody (clone S100A16/7412). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

Quick Database Links

UniProt

Q96FQ6