S100A10 Antibody (Center)

• Catalog Number: orb1928117
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21022
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Porcine, Rabbit
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:8000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 11203 Da
• Target: This S100A10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the Central region of human S100A10.
• UniProt ID: P60903
• NCBI: NP_002957.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Protein S100-A10, Calpactin I light chain, Calpact
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

S100A10 Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma reacted with S100A10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-S100A10 Antibody (Center) at 1:8000 dilution. Lane 1: A431 whole cell lysate. Lane 2: human lung lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 11 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.