S100A1 Antibody

• Catalog Number: orb402952
• Category: Antibodies
• Description: S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain, but not on the beta-chain, of S100. This antibody can be used to localize S-100A in various tissue sections. S100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A1/1942
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Human recombinant S100A1 full length protein was used as the immunogen for this S100A1 antibody.
• Dilution range: Immunohistochemistry (FFPE-not suitable for frozen tissues): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This S100A1 antibody is available for research use only.
• UniProt ID: P23297
• Storage: Store the S100A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the S100A1 antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human melanoma using S100A1 antibody