rVes v 5 (Vespula vulgaris 5.0101) Antibody

SKU: orb1088528

Description

rVes v 5 (Vespula vulgaris 5.0101) Antibody

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, WB
• Application Notes:
  Recombinant protein rVes v 5 is expressed in S2 cells (Drosophila). DNA sequence encoding 218 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 24,9 kDa.

Key Properties

• Purification: Purified by sequential steps of ion exchange and affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Recombinant allergen rVes v 5 purity verification. 5 µg of rVes v 5 with > 95 % purity checked by Coomassie Brilliant Blue stained SDS-PAGE.

Flow cytometry dot-plot staining pattern of rVes v 5 recombinant allergen stimulated human peripheral whole blood lymphocytes and basophils of a proven allergic donor stained using anti-human CD63 (MEM-259) FITC and anti-human CD203c (NP4D6) PE antibodies of BasoFlowEx kit.

Reactivity of rVes v 1, rVes v 2, and rVes v 5 with sIgE antibodies in plasma a patient with confirmed presence (routine clinical test) of specific IgE antibodies to common wasp venom allergen extract. Western blotting analysis was performed on recombinant allergens rVes v 1, rVes v 2, and rVes v 5 mixed and heated (100°C, 5 min) with reducing SDS loading buffer. Sample was resolved using 15% Tris-glycine gel electrophoresis. Nitrocellulose membrane blot was stained with Ponceau S to verify protein transfer. After thorough washing and blocking steps, the membrane was incubated with plasma of an allergic patient (1:10). Captured rVes v 1, rVes v 2, and rVes v 5 sIgE antibodies were detected by biotinylated anti-human IgE secondary antibody (2 μg/ml) followed by IRDye 800CW Streptavidin (1:5000).

Quick Database Links

UniProt

Q05110

NCBI Gene ID

35