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Item 1 of 3
RUNX3 Antibody (Center)
Catalog Number: orb1935595
| Catalog Number | orb1935595 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB34382 |
| Tested applications | IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 44356 Da |
| Target | This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3. |
| UniProt ID | Q13761 |
| NCBI | NP_004341.1, NP_001026850.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Runt-related transcription factor 3, Acute myeloid Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody (Center). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
RUNX3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.
