RUNX1 Antibody (S276)

SKU: orb1931439

Description

RUNX1 Antibody (S276)

Research Area

Cancer Biology, Immunology & Inflammation, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:10-50, FC - 1:10-50
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 48737 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

AML1, CBFA2

RUNX1 Antibody (S276) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of RUNX1 Antibody (S276) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

RUNX1 Antibody (S276) western blot analysis in U-937 cell line lysates (35 ug/lane). This demonstrates the RUNX1 antibody detected the RUNX1 protein (arrow).

All lanes: Anti-RUNX1 Antibody (S276) at 1:2000 dilution. Lane 1: SW620 whole cell lysate. Lane 2: MOLT-4 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

Q01196