RUFY1 Antibody

• Catalog Number: orb1248995
• Category: Antibodies
• Description: RUFY1 Antibody
• Target: RUFY1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-QKNEAITSFEGKT
• UniProt ID: Q96T51
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 85kDa band observed in lysates of cell line HeLa (calculated MW of 79.8kDa according to NP_079434.3). Recommended concentration: 0.05-0.2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: RUFY1, RUN and FYVE domain containing 1, FLJ22251,
• Note: For research use only
• NCBI: NP_001035541.1, NP_079434.3

orb1248995 (0.05 ug/ml) staining of HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248995 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer Ph 6, AP-staining.