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Catalog Number | orb769959 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Rsk-1/2/3/4 (phospho-Ser221/227/S218/232) |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human RSK1/2/3/4 around the phosphorylation site of Ser221/227/S218/232. AA range:191-240 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 85 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-RPS6KA1 antibody, anti-MAPKAPK1A antibody, an Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody.
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using RSK1/2/3/4 (Phospho-Ser221/227/S218/232) Antibody. The lane on the right is blocked with the phospho peptide.