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Item 1 of 5
RRAGD Antibody (Center)
Catalog Number: orb1930740
| Catalog Number | orb1930740 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB25579 |
| Tested applications | FC, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 45588 Da |
| Target | This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD. |
| UniProt ID | Q9NQL2 |
| NCBI | NP_067067.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | Ras-related GTP-binding protein D, Rag D, RagD, RR Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
RRAGD Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RRAGD Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Western blot analysis of RRAGD Antibody (Center) in MDA-MB435 cell line lysates (35 ug/lane). RRAGD (arrow) was detected using the purified Pab.
Western blot analysis of RRAGD Antibody (Center) in mouse cerebellum tissue lysates (35 ug/lane). RRAGD (arrow) was detected using the purified Pab.
Anti-RRAGD Antibody (Center) at 1:1000 dilution + 293T/17 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 46 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
