RPS3A Antibody

• Catalog Number: orb1257271
• Category: Antibodies
• Description: RPS3A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human RPS3A (NP_000997.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 35kDa
• Target: RPS3A
• UniProt ID: P61247
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: 40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RPS3A antibody (orb1257271) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using RPS3A Antibody (orb1257271) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF-7 cells using RPS3A antibody (orb1257271). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 3 ug RPS3A antibody (orb1257271). Western blot was performed from the immunoprecipitate using RPS3A antibody (orb1257271) at a dilution of 1:500.