RPS15A Antibody (N-term)

• Catalog Number: orb1930808
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB24215
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Mouse, Rabbit, Rat, Zebrafish
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 14840 Da
• Target: This RPS15A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human RPS15A.
• UniProt ID: P62244
• NCBI: NP_001025180.1, NP_001010.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: 40S ribosomal protein S15a, RPS15A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

RPS15A Antibody (N-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

RPS15A Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS15A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

All lanes: Anti-RPS15A Antibody (N-term) at 1:1000 dilution. Lane 1: A549 whole cell lysate. Lane 2: K562 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.