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Catalog Number | orb1787986 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | 4-38 aa |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Conjugation | Unconjugated |
MW | 17040 Da |
Target | This RPS15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-38 amino acids from the N-terminal region of human RPS15. |
UniProt ID | P62841 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | 40S ribosomal protein S15, RIG protein, RPS15, RIG Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of HepG2 cells using RPS15 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with RPS15 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-RPS15 Antibody (N-term) at 1:1000 dilution. Lane 1: U-87 MG whole cell lysate. Lane 2: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 17 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
FC, IHC-P, WB | |
Mouse, Porcine, Rabbit, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |