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Catalog Number | orb1256816 |
---|---|
Category | Antibodies |
Description | RPLP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-114 of human RPLP1 (NP_000994.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 17kDa |
Target | RPLP1 |
UniProt ID | P05386 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
Alternative names | 60S acidic ribosomal protein P1, RPLP1, RRP1 Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using RPLP1 antibody (orb1256816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human stomach using RPLP1 antibody (orb1256816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using RPLP1 antibody (orb1256816) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPLP1 antibody (orb1256816) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using RPLP1 antibody (orb1256816). Blue: DAPI for nuclear staining.
ELISA, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat, Zebrafish | |
Polyclonal | |
Unconjugated |
IHC-P | |
Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Rat |
IHC-P | |
Bovine, Canine, Gallus, Human, Porcine |
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