RPL9 Antibody

SKU: orb1260325

Description

RPL9 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-192 of human RPL9 (NP_000652.2).
• Target: RPL9
• Molecular Weight: Observed: 24kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

RPL9, L9, NPC-A-16

Western blot analysis of extracts of various cell lines, using RPL9 antibody (orb1260325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat brain using RPL9 Antibody (orb1260325) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using RPL9 Antibody (orb1260325) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF-7 cells using RPL9 antibody (orb1260325). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

RPL9

UniProt

P32969