RPL7 Antibody

• Catalog Number: orb1257245
• Category: Antibodies
• Description: RPL7 Antibody
• Target: RPL7
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human RPL7 (NP_000962.2).
• UniProt ID: P18124
• MW: Observed: 29kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: L7, humL7-1, 60S ribosomal protein L7, large ribos
• Research Area: Immunology & Inflammation
• Note: For research use only

Western blot analysis of extracts of various cell lines, using RPL7 antibody (orb1257245) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunofluorescence analysis of L929 cells using RPL7 Polyclonal Antibody (orb1257245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.