RPL6 Antibody (N-term)

SKU: orb1788462

Description

Purified Rabbit Polyclonal Antibody (Pab)

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, FC: 1:25
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This RPL6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-35amino acids from the N-terminal region of human RPL6. Antigen Region: 2-35 aa.
• Target: RPL6
• Molecular Weight: 32728 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

60S ribosomal protein L6, Neoplasm-related protein C140, Tax-responsive enhancer element-binding protein 107, TaxREB107, RPL6, TXREB1

Western blot analysis of lysates from Hela, NCI-H1299 cell line (from left to right), using RPL6 Antibody (N-term). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Flow cytometric analysis of Jurkat cells using RPL6 Antibody (N-term) (green) compared to an isotype control of rabbit IgG (blue). Diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded H. gastric carcinoma section using RPL6 Antibody (N-term). NA was diluted at 1: 100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Fluorescent image of HeLa cells stained with RPL6 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

Gene Symbol

RPL6

UniProt

Q02878