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RPL34 Antibody (Center)
Catalog Number: orb1936847
| Catalog Number | orb1936847 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB32974 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Porcine, Rabbit |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 13293 Da |
| Target | This RPL34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-66 amino acids from the Central region of human RPL34. |
| UniProt ID | P49207 |
| NCBI | NP_000986.2, NP_296374.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | 60S ribosomal protein L34, RPL34 Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-RPL34 Antibody (Center) at 1:2000 dilution. Lane 1: A375 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lane 4: U-2OS whole cell lysate. Lane 5: mouse brain lysate. Lane 6: NIH3T3 whole cell lysate. Lane 7: rat liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 13 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RPL34 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPL34 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
RPL34 Antibody (Center) western blot analysis in A375 cell line lysates (35 ug/lane). This demonstrates the RPL34 antibody detected the RPL34 protein (arrow).
