RPL23 Antibody

• Catalog Number: orb1248548
• Category: Antibodies
• Description: RPL23 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Porcine, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: KECADLWPRIASN
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of Human Colorectal Cancer and of cell lines NIH3T3 and HeLa (calculated MW of 14.9kDa according to NP_000969.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Liver. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 16kDa
• Target: RPL23
• UniProt ID: P62829
• NCBI: NP_000969.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RPL23, ribosomal protein L23, L23, MGC111167, MGC1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of Human Colorectal Cancer and of cell lines NIH3T3 and HeLa (calculated MW of 14.9kDa according to NP_000969.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Liver. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248548 (0.1 ug/ml) staining of Human Colorectal Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248548 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248548 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.