RPL23 Antibody

SKU: orb1248548

Description

RPL23 Antibody

Research Area

Cancer Biology, Signal Transduction

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 16kDa band observed in lysates of Human Colorectal Cancer and of cell lines NIH3T3 and HeLa (calculated MW of 14.9kDa according to NP_000969.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Liver. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: KECADLWPRIASN
• Target: RPL23
• Molecular Weight: Approx 16kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Disclaimer: For research use only

Alternative Names

RPL23, ribosomal protein L23, L23, MGC111167, MGC117346, MGC72008, rpL17, 60S ribosomal protein L17, 60S ribosomal protein L23, OTTHUMP00000164151, OTTHUMP00000164153, ribosomal protein L17

orb1248548 (0.1 ug/ml) staining of Human Colorectal Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248548 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1248548 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RPL23

UniProt

P62829

RefSeq (Protein):NP_000969.1