RPL21 Antibody

SKU: orb1260028

Description

RPL21 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human RPL21 (NP_000973.2).
• Target: RPL21
• Molecular Weight: Observed: 18kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

HYPT12, L21, 60S ribosomal protein L21, large ribosomal subunit protein eL21

Western blot analysis of extracts of various cell lines, using RPL21 antibody (orb1260028) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat brain using RPL21 antibody (orb1260028) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using RPL21 antibody (orb1260028) at dilution of 1:200. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

RPL21

UniProt

P46778