RPGR Antibody (C-term)

• Catalog Number: orb1937647
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31028
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 113387 Da
• Target: This RPGR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-772 amino acids of human RPGR.
• UniProt ID: Q92834
• NCBI: NP_001030025.1, NP_000319.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: X-linked retinitis pigmentosa GTPase regulator, RP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-RPGR Antibody (C-term) at 1:1000 dilution + human liver lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 113 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RPGR Antibody (C-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the RPGR antibody detected the RPGR protein (arrow).

RPGR Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPGR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.