RPA32 Antibody / Replication Protein A2

• Catalog Number: orb639746
• Category: Antibodies
• Description: Recognizes a protein of 34kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70kDa, 32-34kDa, and 11-14kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: RPA2/3140R
• Tested applications: WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: A recombinant human full-length Replication protein A2 protein was used as the immunogen for this recombinant RPA32 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant RPA32 antibody is available for research use only.
• UniProt ID: P15927
• Storage: Store the recombinant RPA32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MCF7 and T47D cell lysate with recombinant RPA32 antibody. Expected molecular weight ~32 kDa.