Roundabout 4 Antibody (N-term)

• Catalog Number: orb1930667
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Roundabout 4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the N-terminal region of human Roundabout 4.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q8WZ75
• MW: 107457 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB21227
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Roundabout homolog 4, Magic roundabout, ROBO4
• Note: For research use only
• NCBI: NP_061928.4

Roundabout 4 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Roundabout 4 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Roundabout 4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Roundabout 4 Antibody (N-term) western blot analysis in 293, K562, HL-60 cell line lysates (35 ug/lane). This demonstrates the Roundabout4 antibody detected the Roundabout4 protein (arrow).