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RORA Antibody (T216)

Catalog Number: orb1938644

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb1938644
CategoryAntibodies
DescriptionAffinity Purified Rabbit Polyclonal Antibody (Pab)
Species/HostRabbit
ClonalityPolyclonal
Clone NumberRB16632
Tested applicationsFC, IF, WB
Predicted ReactivityMouse
ReactivityHuman
IsotypeRabbit IgG
Antibody TypePrimary Antibody
Dilution rangeIF: 1:10~50, WB: 1:1000, WB: 1:500, FC: 1:25
Form/AppearancePurified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
ConjugationUnconjugated
MW58975 Da
TargetThis RORA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 193-222 amino acids from human RORA.
UniProt IDP35398
NCBINP_002934.1, NP_599022.1, NP_599023.1, NP_599024.1
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Alternative namesNuclear receptor ROR-alpha, Nuclear receptor RZR-a
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NoteFor research use only
Expiration Date12 months from date of receipt.
RORA Antibody (T216)

Anti-RORA Antibody (T216) at 1:500 dilution + Human brain lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 59 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RORA Antibody (T216)

Fluorescent confocal image of Hela cell stained with RORA Antibody (T216). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RORA primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RORA immunoreactivity is localized to Nucleus significantly.

RORA Antibody (T216)

Overlay histogram showing HepG2 cells (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

RORA Antibody (T216)

Western blot analysis of anti-RORA Antibody (T216) in A2058 cell line lysates (35 ug/lane). RORA (arrow) was detected using the purified Pab.