ROR2 Antibody (C-term)

• Catalog Number: orb1928995
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01510
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000-1:2000, WB: 1:1000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 104757 Da
• Target: This ROR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 915-943 amino acids from the C-terminal region of human ROR2.
• UniProt ID: Q01974
• NCBI: NP_004551.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Tyrosine-protein kinase transmembrane receptor ROR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ROR2 (arrow) using rabbit polyclonal ROR2 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ROR2 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

All lanes: Anti-ROR2 Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: T47D whole cell lysate. Lane 4: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-ROR2 Antibody (C-term) at 1:1000-1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: NCCIT whole cell lysate. Lane 4: SH-SY5Y whole cell lysate. Lane 5: T47D whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 105 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.